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Ex-vivo Organ Perfusion: Application to the study of skeletal muscle in rats

#Skeletalmuscle #Rat #Productusage



In the organ culture system (organ bioreactor), different organs can be perfused by selecting different parts of the organ chambers.


For example, it is possible to purchase one main controller of a bioreactor, as a shared device for a lab, and also purchase an individual/exclusively designed chamber unit for specific samples.


In this article, we introduce a case study of a successful perfusion culture of rat skeletal muscle for 7 days.


In the field of skeletal muscle research, applying the Ex-vivo perfusion system will help to achieve a better result by filling the gap between animal experiments and cell experiments.



Overview

The femoral skeletal muscles were harvested from the rats and cultured into the organ culture system and, after 7 days of perfusion, high perfusion rates were maintained.



Background and aim

To enable analysis of skeletal muscle isolated from individual animals, we aimed to maintain the activity of the skeletal muscle in a perfusion culture under analyzable conditions, which cannot be analyzed in individual animals.


The perfusion rate was defined by the following equation:

Perfusion rate = return volume from vein ( g ) / pumped volume ( g )



Result

We successfully perfused the cells for 5 days, maintaining a perfusion rate of around 20% (Fig .1).


This allowed for perfusion culture with return fluid from the vein to be analyzed.


As a result of this experiment, a perfusion culture was achieved that enabled return fluid from the vein to be analyzable.



References.

Sano, K. et al. J Artif Organs (2019) link



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