Have you ever felt the following needs during the study of the small intestine?
"The results are different when you are working with cells and animals. Do not know why."
"I want to perform absorption model experiments in the small intestine only."
"I want to control drug dosage to the small intestine directly."
"I want to sample directly from the superior mesenteric vein."
The small intestine chamber of our "Organ Culture System" allows you to run small intestinal perfusion experiments in rats surprisingly easily.
In this article, I would like to introduce an example of the experiment.
For graphs and tissue slices images can be downloaded here.
Application Note：Rat small intestine
The document of the case of rat small intestine perfusion experiment.
Overview
The small intestine was removed from the rat and after 20 hours of perfusion culture in the Organ Culture System, it showed peristalsis and maintained its perfusion rate.
Background and aim
To allow analysis in the isolated small intestine, which wouldn’t be possible in individual animals, we aimed to achieve a perfusion culture that remained active and ready for analysis.
The perfusion rate was defined by the following equation.
Perfusion rate = return volume from vein (g)/infused volume (g)
Result
After 20 hours of perfusion culture, peristalsis and perfusion were observed, while a perfusion rate of around 40% is maintained. It was possible (Fig. 1).
In the perfused tissue sections, smooth muscle and mucosal-like tissue were maintained (Fig. 2).
These results suggest that the tissue section (after perfusion) remained active and the return fluid from the vein could be analyzed (Fig.2).
The figures are available for download.
References
Sano, K. et al. J Artif Organs (2019) link
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