[Perfusion culture] We will teach you the correct washing method in detail.
Tokaihit- 7 days ago
- 4 min read
Updated: 6 days ago
Hello.
Recently, the author has been focusing on perfusion experiments.
That's what I think every time I finish an experiment...
it is,
"Someone please do the cleaning...!!"
However, he shook his head, changed his mind, and reluctantly began cleaning.
(I'm sure you've all had this experience...)
because,
In perfusion experiments, washing after the experiment is
Because it's "absolutely essential."
This article is about the post-experiment cleaning method, which is important for perfusion experiments.
I will introduce all the methods for cleaning an irrigation system based on examples of cleaning that I have actually performed.
table of contents
1. The importance of cleaning
2. The most important thing is "tube cleaning"
3. What happens inside the pump?
4. Chamber cleaning
5. Summary
1. The importance of cleaning
The only reason cleaning is necessary is to perform "precise experiments."
In perfusion experiments, the device serves as a flow path for transporting perfusion fluids such as culture medium and blood.
There will be lots of tubes.
In experiments, various culture media are poured into these tubes.
If the tube is not washed properly after an experiment, components of the culture medium will remain in the tube.
Then, in the next experiment, the ingredients mixed with new medium...
Naturally, there is a possibility of contamination.
(Of course, if contamination occurs, all the time and effort spent will be for nothing...)
This is why cleaning is essential to conducting accurate experiments.
In addition, the tube is the part that comes into contact with the medium the most in a perfusion experiment, so
You will need to clean it more thoroughly.
2. The most important thing is "tube cleaning"
So how do you clean the tubes? I'll explain step by step using my own cleaning examples.
To make cleaning easier, remove the tubing from the pump unit and chamber before
Proceed with cleaning in the following steps:
1) Use a syringe to pass water through the tube 2-3 times.
At this point, most of the dirt caused by the medium will be washed away with water.
2) Use a syringe to pass 70% ethanol through the tube 2-3 times.
Sterilize and clean the inside of the tube with ethanol.
3) Pass air through the tube to remove any liquid, then allow the tube to dry.
When sterilizing with EOG, be sure to dry the tube. If liquid remains in the tube, the EOG gas may dissolve in the liquid and cause toxicity to cells.
First, remove as much liquid as possible from the tube by passing air through it.
Then dry thoroughly using a dryer, etc.
In addition, in the case of autoclave sterilization, drying is performed after sterilization, so step 3) is not necessary.
3. What happens inside the pump?
There are also tubes inside the pump, which of course need to be cleaned as well.
However, if you are using a roller pump with a pump head,
You need to be a little careful.
In this case,
Due to the structure of the pump, it is not possible to pass liquid through it using a syringe.
Therefore, water and 70% ethanol are passed through using a pump .
1) Connect the tubing to the pump head to be cleaned and attach the pump head to the pump unit.
2) Activate the pump and pass water, then 70% ethanol through the tube, 2-3 times each.
3) Pass air through the tube to remove any liquid, then allow the tube to dry.
This completes the cleaning of the tubes inside the pump.
If you do not have a pump head, follow the same cleaning procedure using a syringe.
4. Chamber cleaning
Once you get this far, all that's left is the finishing touches.
Fill the chamber with household dish soap and a sponge.
Use it to clean.
I make sure to wash gently with foam to avoid scratching the surface.
(Custom-made chambers are expensive...)
After washing, tumble dry or
Dry at room temperature.
If you are sterilizing using an autoclave,
After sterilization, dry it and clean up is complete!
5. Summary
This time, we introduced the important post-experiment cleaning method for perfusion culture.
To summarise,
■ Tube cleaning is essential for perfusion culture
■ Pass water and 70% ethanol through the tube 2-3 times.
■ If the pump head is attached, operate the pump to clean the inside of the pump.
This means that.
What did you think?
If you prepare everything disposable for your experiment, there is no need to clean it.
In that case, the costs would inevitably increase, and many researchers would find this difficult.
However, don't worry, if you clean it properly there will be no contamination!
(I've been doing perfusion experiments for 4 years now and have never had a single contamination incident! )
I hope this article will be helpful to those who are interested in perfusion culture or have questions about cleaning methods.
I am very happy.
Thank you for staying with us until the end.
The perfusion system introduced this time is here ↓
■ Constant pressure/constant flow rate liquid delivery unit BPU
While monitoring the flow path pressure
Constant pressure perfusion can be achieved.
Tokai Hit offers the best combination of perfusion equipment from its lineup and combines its development technologies.
We are offering solution proposals. If you have any requests, please contact us using the form below.
*Machine Translation
This site uses machine translation. In some cases, the translation may be inaccurate or unintended. PDF and image files may not be translated. In the case of any discrepancy between the Japanese site and English site, the original Japanese site shall prevail.
Comments